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A simple, precise, specific and accurate high performance thin layer chromatographic method has been developed for the
simultaneous determination of olmesartan medoxomil and indapamide in pharmaceutical dosage form. The separation was
carried out on Merck HPTLC aluminium plates of silica gel 60F
254
, using toluene: chloroform: ethanol (4:4:1
v/v
) as the mobile
phase. HPTLC separation of the two drugs followed by densitometric measurement was carried out in the absorbance mode at
254 nm. The drugs were resolved with Rf values of 0.15 and 0.47 for olmesartan medoxomil and indapamide, respectively. The
linear regression analysis data for the calibration plots showed good linear relationship with r value 0.99930 and 0.99660 for
olmesartan medoxomil and indapamide respectively, in the concentration range of 100 to 700 ng/spot for olmesartan and 100
to 600 ng/spot for indapamide. The method was validated according to the ICH guidelines with respect to accuracy, precision,
specificity and robustness. The limit of detection and quantitation were 100 and 300 ng/spot respectively for olmesartan and 100
and 300 ng/spot for indapamide. The proposed developed HPTLC method can be applied for identification and quantitative
determination of olmesartan medoxomil and indapamide in bulk drugs and pharmaceutical dosage form.
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