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Development and validation of analytical method for simultaneous estimation of paracetamol and thiocolchicoside by RP-HPLC in bulk and pharmaceutical dosage form
International Conference on Pain Research & Management
Chiragkumar M Patel
Shivam Pharmaceutical Studies and Research Centre, India
Posters & Accepted Abstracts: J Pain Relief
Abstract
A simple, precise and accurate HPLC method has been developed and validated for assay of combined dosage form of paracetamol and thiocolchicoside in commercial pharmaceutical dosage form. Reversed-Phase High Performance Liquid Chromatographic (RP-HPLC) analysis was performed on a BDS Hypersil C18, 250 mm��4.6 mm, 5 �¼ (particle size) and Thermo Scientific column using potassium di-hydrogen phosphate: methanol (40:60, v/v) as eluent. The flow rate of the mobile phase was adjusted to 1.0 ml/min and the injection volume was 20 �¼l. Detection performed at 247 nm. The retention time of paracetamol and thiocolchicoside were found to be 3.27 and 5.50 respectively. The method was validated for linearity, precision, accuracy and robustness. Response was a linear function of drug concentration in the range with 250-750 �¼g/ml for paracetamol and 1-3 �¼g/ml for thiocolchicoside. Intra-day and inter-day precision were determined. Accuracy of paracetamol and thiocolchicoside was found between 99-100%. All analytical validation parameters were determined by following the ICH guidelines and its limit. The developed method proclaimed to be precise and robust for the estimation of paracetamol and thiocolchicoside in their combined dosage form.Biography
Email: chipatel459@gmail.com
