Journal of Biotechnology & Biomaterials
Open Access

Abstract

Significant efforts are being given throughout the world for an ideal solution on orthopaedic diseases and trauma which lead to a new research in biomedical field and bone tissue engineering. Mesoporous Bioactive Glass (MBG) can be used as a combined function of controlled local antibiotic delivery as well as its in-built property to form hydroxyapatite layer helps in bone restoration. In the present investigation MBG nanopowders of SiO2-CaO-P2O5 system have been successfully fabricated by three different methods by using structure directing agent Pluronic P123 via hydrothermal method (MBHM), an ionic surfactant CTAB (hexadecyltrimethylammonium bromide) (MBCTAB) and through wet chemical method (MBWC) using citric acid and polyethylene glycol. A comparative study of phase, morphology and textural properties of all MBG nanopowders were accomplished by DTA-TGA, XRD and FTIR spectroscopy, DLS, FESEM, BET and TEM. In vitro bioactivity test were studied by immersing MBG nanopowders in SBF for 24 and 48 hours with the intention to examine the hydroxyapatite layer over the surface of glass powders. ICP-AAS confirmed increase in Ca2+ and PO4 3- ions in MBG samples and other characterizations like FTIR, XRD and FESEM confirmed the HAp deposition over the MBG nanopowders. Cytotoxicity evaluation was also done to investigate the cell growth and viability of NIH3T3 cell line on the MBG powdered pellets. These observations proposed that MBHM650 and MBWC650 could be suitable as filler material basically in dental and orthopaedics applications whereas high surface area of MBCTAB650 powders can be more useful for delivering pharmaceuticals agents in vitro/in vivo.

Biography

Email: akrityanand1@gmail.com biswa.kundun@gmail.com