Journal of Biotechnology & Biomaterials
Open Access

Abstract

Forty-five rhizofungal isolates were isolated, identified and characterized from eleven herbicides polluted-soil. Among the isolates, ten fungal species proved to be most potent and promising ones in herbicides tolerance. The herbicides exhibited sever and dramatic effect and modulation on fungal DNA and protein represented in DNA and protein profile. Severely loss in the total soluble cell ions (SCI) and total cell protein percentage (TCPC) concentrations was observed. The loss of SCI by glyphosate, Aspergillusflavus (86.30%)was the most affected one, followed by Penicilliumspiculisporus (76.30%), Penicilliumverruculosum(64.40%) and Alternariatenuissima (64%), respectively. For Pendimethalin, Alternariatenuissima (54.01%) was the most affected fungi. For Diclofop-methyl, Penicilliumspiculisporus (74.20%) was the most affected fungi. The loss of TCPC by Glyphosate, Alternariatenuissima (64.71%) was the most effected fungi, followed by Penicilliumspiculisporus (57.14%), respectively. For Pendimethalin, A. terreus (54.29%) was the most affected fungi. For Diclofop-methyl, Penicilliumspiculisporus (60%), followed by Alternariatenuissima (58.82%), Aspergillustamarii (55.56%), respectively. The results proved sever reductions and alteration in protein, SCI, TCPC and DNA in fungal strains exposed to these herbicides which may reflex a degree of tolerance occurred during the assimilation of those toxic compounds from the pesticides polluted-soil.

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