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Determination of parabens in cosmetic samples using fabric phase sorptive extraction (FPSE) by high-performance liquid chromatography and ultraviolet detection
7th International Conference and Exhibition on Analytical & Bioanalytical Techniques
Ashok Kumar Malik, Ramandeep Kaur, Heena, Abuzar Kabir and Kenneth G Furton
Punjabi University, India Florida International University, USA
Posters & Accepted Abstracts: J Anal Bioanal Tech
Abstract
A simple, fast and sensitive analytical method using fabric phase sorptive extraction (FPSE) followed by high performance liquid chromatography ultraviolet detection (HPLC-UV) has been developed and validated for the extraction of five parabens namely ethyl, methyl, propyl, butyl and benzyl paraben. Parabens are esters of p-hydroxybenzoic acid which are commonly used as preservatives in cosmetic products. Various factors affecting the performance of FPSE technique such as extraction time, eluting solvent, elution time and pH of the sample matrix were optimized. Determination was performed using reversed stationary phase C18 column with wavelength 254 nm. Separation was performed using mobile phase acetonitrile: water (63:37; v/v) at an isocratic flow rate of 1.0 mL/min. The calibration curves of the target analytes were prepared with good correlation coefficient values (R2>0.992). The limit of detection (LOD) values range from 0.192-0.225 ng/mL and the developed method was applied successfully for the analysis of parabens in various cosmetic samples such as rose water, deodorant, serum and cream with extraction recoveries found in between 88% to 114%.Biography
Email: malik_chem2002@yahoo.co.uk
