Journal of Biotechnology & Biomaterials
Open Access

Abstract

Heavy metal pollution is an environmental problem of worldwide concern because most of them can be toxic even at low concentrations. Industrialized societies are responsible for increasing environmental contamination by trace metals produced as wastes from industrial and agricultural processes and household activities. In the present study isolation, identification and determination of the minimum inhibitory concentration (MIC) of Chrysobacterium indolegenes HMT 47 was done. The strain was isolated from rhizospheric soil of plants growing in mine spoil of Zawar mines, Udaipur, Rajasthan, India. Isolation was done on nutrient agar supplementated with 0.5 mM of Lead Nitrate by standard Pour Plate Method. The isolate was then identified on the basis of morphological and biochemical characteristics which was further confirmed by 16S rRNA gene sequencing. The amplification reaction was proformed using 16S rRNA gene specific universal primers namely 27F and 1492R. The minimum inhibitory concentration of Chrysobacterium indolegenes HMT 47 against lead was determined on nutrient agar supplementated with varying concentrations of lead nitrate ranging from 100 to 1100 �¼g/ml. The MIC of the isolate against lead nitrate was found to be 1000 �¼g/ml. The lead tolerant bacterium can proved to be a potential bioremediation tool for in situ stabilization and remediation of Lead contamination sites.

Biography

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