Journal of Biotechnology & Biomaterials
Open Access

Abstract

The increased anthropogenic activities such as incomplete combustion of coal, wood and petroleum, also the road accidents, mining accidents, shipping transportation etc., have a dramatically effects the soil pollution with PAHs. The priority pollutants are of great concern in the environment. The microorganisms play a critical role in the removal of these toxic and mutagenic poly aromatic hydrocarbons. The hydrocarbon degrading microbial strain was purchased from IMTECH, Chandigarh, India. The GC-MS (Shimadzu model QP-2010 plus, column-Rtx-5MS, 30 meter��0.25 mm i.d��0.25 um film thickness) was used to separate and identify the aliphatic and aromatic fractions of oil present in the 2T engine oil. The morphological characterization (Gram�s staining), biochemical characterization (catalase test, IMVIC test, starch hydrolysis etc.) and molecular characterization (16s RNA sequencing) was performed. The maximum degradation of the P. dendritiformis strain was obtained 63% after 28 days in the shaker fermentation process. The conversion of high molecular weight compound to the low molecular weight compounds indicated the partial degradation of Nonacosane and Tetracontane by the P. dendritiformis strain T168 by gravimetric method in 35 days of fermentation.

Biography

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