Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers
Google Scholar citation report
Citations : 3314
Journal of Biotechnology & Biomaterials received 3314 citations as per Google Scholar report
Indexed In
- Index Copernicus
- Google Scholar
- Sherpa Romeo
- Open J Gate
- Genamics JournalSeek
- Academic Keys
- ResearchBible
- China National Knowledge Infrastructure (CNKI)
- Access to Global Online Research in Agriculture (AGORA)
- Electronic Journals Library
- RefSeek
- Hamdard University
- EBSCO A-Z
- OCLC- WorldCat
- SWB online catalog
- Virtual Library of Biology (vifabio)
- Publons
- Geneva Foundation for Medical Education and Research
- Euro Pub
- ICMJE
Useful Links
Recommended Journals
Related Subjects
Share This Page
In Association with
Decreased Camptothecin sensitivity of cancer stem-like cell population correlates with phosphorylation state of DNA topoisomerase-I
6th World Congress on Biotechnology
Amit Roy1,2 and Birgitta R Knudsen2
1National Institute of Pharmaceutical Education & Research, India 2Aarhus University, Denmark
ScientificTracks Abstracts: J Biotechnol Biomater
Abstract
Cancer stem cells are characterized by indefinite self-renewal potential and capacity to differentiate into progeny cells that constitute the bulk of tumor. Moreover, they are frequently refractory to anti-cancer therapy. The present study addressed the molecular mechanism behind resistance towards the anti-tumor drugs like camptothecin. The CD44+ and CD44- subpopulations of colorectal cancer cell line Caco-2 were analyzed separately for their sensitivities to camptothecin. Cancer stem-like cell subpopulation CD44+ is significantly less sensitive towards treatment with camptothecin than CD44- cell subpopulation. Topoisomerase-I from the two subpopulations were differentially phosphorylated in a manner that appeared to determine the drug sensitivity and activity of the enzyme. This finding was further supported by the fact that phosphorylation of topoisomerase-I in CD44+ cell extract by protein kinase CK2 converted the enzyme to a camptothecin sensitive, more active form mimicking topoisomerase-I in extracts from CD44- cells. Therefore, the reduced activity level and insensitivity towards camptothecin could be ascribed directly to hypo-phosphorylation of topoisomerase-I in the cancer stem-like cell subpopulation. This is the first example of the phosphorylation state of topoisomerase-I modifying both activity and camptothecin sensitivity of the enzyme. In conclusion, to our knowledge this is the first report of an altered topoisomerase-I activity in cancer stem-like cells relative to progeny cells of a cancer cell line that can explain the camptothecin resistant phenotype of the cancer stem cell subpopulation.Biography
Amit Roy has completed his PhD in 2009 from CSIR-Indian Institute of Chemical Biology, Kolkata, India and Postdoctoral studies from Aarhus University, Denmark. He is now an Assistant Professor at Department of Biotechnology, National Institute of Pharmaceutical Education & Research, India. He has published more than 21 research papers in reputed peer-reviewed international journals. His PhD work was highly recognized by Indian National Science Academy (INSA) and he has been awarded the INSA Young Scientist Medal and Professor LSS Kumar Memorial Award in the year 2011. Apart from that, he got many awards including Best Award, Best Presentation Awards, etc.
Email: amitavik@gmail.com
