Journal of Biotechnology & Biomaterials
Open Access

Abstract

Anovel bacterial strain named GSM3 capable of degrading Reactive Violet 5 was successfully isolated from soil sample collected from Ammu Dyeing Industry, Bellary, India. The strain was identified as a member of Pseudomonas aeruginosa based on morphological features, physiological and biochemical characteristics, and phylogenetic analysis based on the sequence of 16S rDNA. The organism showed 100% decolorization of sulfonated azo dye Reactive Violet 5 100 mg L-1 within 24 h, while maximally it could decolorize 800 mg L-1 of dye within 114 h with 63% decolorization in a carbon free mineral salts medium supplemented with 0.1% (w/v) of yeast extract as a cosubstrate under static condition. The isolate has shown good decolorization activity over a wide range of pH from 5.0 to 9.0 and temperature 25oC to 40oC. However, highest decolorization was observed at pH 7.0 and temperature at 37oC under static conditions. Strain showed good dye decolorization activity in the presence of 5% NaCl. The culture exhibited significant ability to decolorize repeated additions of dye, with decrease in time up to 12 h at seventh dye aliquot addition. The organism was also capable of decolorizing five other reactive dyes. The dye decolorization and biodegradation was monitored by UV-visible spectroscopy, TLC and FT-IR spectroscopy. Thus, the isolate GSM3 has the potential to be employed for the treatment of dye contaminated textile wastewaters.

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