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In Association with
Contribution of efflux pump in drug resistance of fluoroquinolone resistant Mycobacterium tuberculosis isolates
6th World Congress on Biotechnology
Richa Kumari, Rajneesh Tripathi, Alok Prakash Pandey, Prince Chaubey, Pallavi Sinha and Shampa Anupurba
Banaras Hindu University, India
Posters-Accepted Abstracts: J Biotechnol Biomater
Abstract
Fluoroquinolones (FQs) are widely used second line anti tuberculosis drug. Extensive use of FQs has increased the incidence of resistance in Mycobacterium tuberculosis. Apart from mutation in the drug target site i.e., gyr A and gyr B the resistance may develop due to active efflux pump. In this study, we evaluated the role of the efflux pumps in fluoroquinolone resistance by using efflux inhibitors carbonyl cyanide m-chlorophenyl hydrazone (CCCP) which acts by decreasing the trans-membrane electrochemical gradient and verapamil, a calcium channel blocker in clinical isolates of M. tuberculosis. A total of 50 fluoroquinolone resistant M. tuberculosis clinical isolates were tested by Resazurin micro titer assay (REMA) to observe the changes in minimum inhibitory concentration (MIC) in presence and absence of efflux inhibitors using standard strain H37Rv. The MIC levels showed 2-8 fold reduction in presence of CCCP (10/50 20%) and verapamil (12/50 24%). Reduction in MIC was observed in (15/50 30%) strains when both the inhibitors were used simultaneously. Our findings suggest that an active efflux pump could be a major contributor in development of resistance against fluoroquinolones. Significant reduction in MIC indicates the involvement of Major Facilitator Super Family (MFS) family and ATP Binding Cassette (ABC) transporters which are inhibited by CCCP and verapamil respectively.Biography
Email: richaimsbhu@gmail.com
