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Construction of recombinant protein of influenza A virus neuraminidase gene expressed in baculovirus
2nd International Conference on Influenza
Masoud Moghaddam Pour, Hossein Keivani, Shahin Masoudi, Seeid Hamid Monavari, Mohammad Najafi and Majid Tebianian
Iran University of Medical Sciences, Iran Razi Vaccine & Serum Research Institute, Iran
Posters & Accepted Abstracts: J Infect Dis Ther
Abstract
Two structural antigens, haemagglutinin (HA) and neuraminidase (NA) are attractive candidates for the development of a genetically engineered vaccine against influenza. Recombinant vaccines are produced by a simple and effective method, although expected to induce an immune response to a specific antigen, remain to be further improved for their high effectiveness. On the other hand, a potent and effective vaccine against influenza should be able to induce both humoral and cellular immune responses. In the present study, the NA gene, which is more stable than the HA one was amplified by Polymerase Chain Reaction (PCR) and then cloned into a eukaryotic expression vector pFastBac HTA. The purity of the expressed NA protein was analyzed on SDS-PAGE electrophoresis. Western blot was carried out to examine the expression of NA using the commercial anti-NA polyclonal antibody. Additionally, an immunofluorescence assay was used to qualitatively assess the antigenicity and biological activity profiles of the recombinant protein, NA, on infected Sf9 cell surface by using immunized rabbit antiserum.Biography
Email: mmoghaddamp@yahoo.com
