Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 3314

Journal of Biotechnology & Biomaterials received 3314 citations as per Google Scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • EBSCO A-Z
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
  • ICMJE
Recommended Journals
Share This Page

Compensatory effects of hOGG1 for hMTH1 in oxidative DNA damage caused by hydrogen peroxide

2nd World Congress on Bio Summit & Molecular Biology Expo

Yuebin Ke and Ziquan Lv

Shenzhen Center for Disease Control and Prevention, China

Posters & Accepted Abstracts: J Biotechnol Biomater

DOI: 10.4172/2155-952X.C1.062

Abstract
This study aimed to investigate the potential compensatory effects of hOGG1 and hMTH1 in the repair of oxidative DNA damage. The hOGG1 and hMTH1 gene knockdown human embryonic pulmonary fibroblast cell lines were established by Lentivirus-mediated RNA interference. The messenger RNA (mRNA) levels of hOGG1 and hM1TH1 were analyzed by the real-time polymerase chain reaction and 8-hydroxy-20-deoxyguanosine (8-oxo-dG) formation was analyzed in a highperformance liquid chromatography-electrochemical detection system. The hOGG1 and hMTH1 knockdown cells were obtained through blasticidin selection. After transfection of hOGG1 and hMTH1 small interfering RNA, the expression levels of the mRNA of hOGG1 and hMTH1 genes were decreased by 97.2% and 96.2%, respectively. The cells then were exposed to 100 mmol/L of hydrogen peroxide (H2O2) for 12 hours to induce oxidative DNA damage. After H2O2 exposure, hMTH1 mRNA levels were increased by 25% in hOGG1 gene knockdown cells, whereas hOGG1 mRNA levels were increased by 52% in hMTH1 gene knockdown cells. Following the treatment with H2O2, the 8-oxo-dG levels in the DNA of hOGG1 gene knockdown cells were 3.1-fold higher than those in untreated HFL cells and 1.67-fold higher than those in H2O2-treated wild-type cells. The 8-oxo-dG levels in hMTH1 gene knockdown cells were 2.3-fold higher than those in untreated human embryonic pulmonary fibroblast cells but did not differ significantly from those in H2O2-treated wild-type cells. Our data suggested that hOGG1 could compensate for hMTH1 during oxidative DNA damage caused by H2O2, whereas hMTH1 could not compensate sufficiently for hOGG1 during the process.
Biography

Yuebin Ke is a Professor of the Shenzhen Center for Disease Control and Prevention and an Adjunct Professor of Life Sciences at Shenzhen University. He has completed his PhD from Huazhong University of Science and Technology and Postdoctoral studies from Virginia Polytechnic Institute and State University. He has published 18 papers in the areas of environmental health and molecular biology.

Email: keyke@szu.edu.cn

Top