Journal of Biotechnology & Biomaterials
Open Access

Abstract

Traditional production of 1,3-propanediol (1,3-PD) involves chemical synthesis from petroleum derivatives compounds. The crisis of crude oil market and environmental concerns facilitate industrial microbiology as an interesting alternative for production of petroleum-based chemicals. Clostridium butyricum is a microorganisms that has been known for decades to have the ability to produce 1,3-propanediol. The fact that it can be produced from crude glycerol (by-product from biodiesel industry) as a carbon source in culture medium is an attractive way to utilize this waste. It is also interesting from envioronmental point of view. The aim of this work was to compare different wild Clostridium butyricum isolates obtained from natural environment in terms of 1,3-PD production on substrate obtained from different industrial sources. Nine isolates were cultured on media containing nine different types of glycerol. Batch cultivations were carried out using 70 g/l concentration of substrate in respect of pure glycerol. Experimental results showed significant differences in 1,3-PD production depending on strain and glycerol type used. It was determined that the best isolates (able to produce 1,3-PD efficiently on different substrate type) were C. butyricum DO 1.6 and C. butyricum JS 3.

Biography

Katarzyna Czaczyk is professor at Department of Biotechnology and Food Microbiology, Specialist in food microbiology and biochemistry analysis and author of numerous thesis, papers and scientific books.