Journal of Infectious Diseases & Therapy
Open Access

Abstract

Increasing demands for seasonal influenza vaccine and the need for faster methods of vaccine production during flu pandemics and the threat posed by highly pathogenic avian influenza viruses, have made cell culture a suitable substrate for influenza vaccine manufacturers. Cell-adapted viruses replicate with high fidelity, which are expected to have potent vaccine immunogenicity. The aim of this study was evaluating MDCK and MDCK-SIAT1 cell lines for their ability to produce the yield of influenza virus. Yields obtained for influenza virus H1N1 grown in MDCK-SIAT1 cell was almost the same level as MDCK; however, H3N2 virus grown in MDCK-SIAT1 showed lower peak of viral titers in comparison with MDCK cells. The optimized MOI to infect the cells on plates and microcarrier was 0.01 and 0.1 for H1N1 and 0.001 and 0.01 for H3N2, respectively. MDCK-SIAT1 cells have the capacity to be considered as an alternative mean to manufacture cell-based flu vaccine, especially for the human strains (H1N1), due to its antigenic stability and high titer of influenza virus production compared to egg inoculation.

Biography

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