Journal of Biotechnology & Biomaterials
Open Access

Abstract

The Nucleotide Binding Site-Leucine Rich Repeat (NBS-LRR) is the disease resistance gene present in plants. One of the major challenges faced by modern agriculture is to achieve not only a satisfactory but also an environment friendly control of plant diseases. Therefore in this work it is aimed to clone NBS-LRR gene from Jatropha to E. coli and use it as the biocontrol agent to overcome these challenges. Jatropha curcus was chosen as the source for NBS-LRR gene because there are about 150 clones of resistance (R) genes present in it. The primer was designed specific to the NBS-LRR gene and it was amplified by Polymerase Chain Reaction (PCR). The amplicon was observed to be 600 bp. It was then eluted for ligation with pGEMT vector. Totally 107 colonies were obtained in blue white screening, out of which 41 were white. Presence of insert was confirmed by PCR confirmation and restriction analysis. The dual culture test was done against Fusarium and activity was compared with Trichoderma and normal E. coli. The transformed E. coli DH5�± strain exhibited 64% inhibition which was more efficient than Trichoderma which showed 61% and normal E. coli with 43% inhibition. Therefore the transformed strain was formulated and can be used as a biocontrol agent.

Biography

Sri Ram A has completed his BTech in Biotechnology from K S Rangasamy College of Technology, Tamil Nadu. He is currently pursuing his MTech in Biotechnology at Amity Institiute of Biotechnology, Noida. He has filed a patent (for the formulation process) for his project entitled “Cloning of NBS-LRR gene and its application as a biocontrol agent”.

Email: sriram.biotek@gmail.com