Journal of Biotechnology & Biomaterials
Open Access

Abstract

Bacterial blight (BB) is caused by Xanthomonas oryzae pv. oryzae has been one of the most serious diseases in rice. Using PCR based allele mining; the present investigation was done to find out novel alleles of Xa27 gene from eight wild rice species and 11 cultivated rice lines. This analysis to find out structural variation and its impact on the phenotypes found maximum variation at nucleotide level in the form of transition. Sequence analysis indicated more variation between cultivated and wild species as compared to variation between wild species of rice. The 8 SNPs and 4 InDels found in alleles from Basmati rice varieties may be the reason for its susceptible nature. All nineteen alleles studied in present investigation, have only one exon in its ORF region. Two motifs such as Protein kinase C phosphorylation site and N-myristoylation site have been identified in all the alleles indicating its role in signal transduction process. The Xa27 alleles have 13 polymorphic sites with nucleotide diversity of 0.01259 and �¸w=0.01114. Further, the study of Ka/Ks ratio suggests that there is Darwinian pattern of selection which leads to divergent evolution of Xa27 gene in nature. Important allelic variants found in this study can be used for developing gene specific DNA markers which can be used in resistance breeding. Besides, various resistant alleles cloned from wild species can be exploited in the effective management of bacterial blight disease after transferring these alleles either alone or in combination in the susceptible genotypes.

Biography

Email: sangeeta10mar@gmail.com