Journal of Biotechnology & Biomaterials
Open Access

Abstract

Seed storage proteins of grain crops meet the major dietary protein requirement of over half of the world population, therefore intensive research on isolation and characterization of these proteins and their genes is an important aspect for improvement of nutritive value in human food and animal feed. These seed storage proteins during seed development are abundant during the mid-maturation period and decline towards maturation. The isolation and complete nucleotide sequencing of 1.7 kb (acc. KM488332) legumin type protein gene was determined using primer walking strategy. BLAST analysis of the sequence revealed >90% homology with legumin gene nucleotide sequences bearing accession numbers D87980, GQ358524 and AY245536. The deduced amino acid sequence from the open reading frame coded for a 64 kDa pre-protein. BLASTp analysis of the sequence revealed 98% homology with 13S globulin seed storage protein from common buckwheat (acc.no.O23878) showing a characteristic barrel domain of the â��cupinâ�� superfamily which comprises the 11S and 7S plant seed storage proteins and germins. Analysis of the deduced amino acid sequence revealed the presence of �± and �² subunit linked ASN-GLU linkage. This gene was cloned into pJET1.2 blunt cloning vector (Fermentas) and further sub-cloned into pCAMBIA1304 through directional cloning strategy which would enable us to characterize its further candidature in research programmes aimed at improvement of the nutritional quality of conventional crops deficient in essential amino acids.

Biography

Email: dlashaihun@hotmail.com