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In Association with
Characterization of guava genotypes using RAPD markers
6th World Congress on Biotechnology
Arumugam Nagaraja1, Banoth Shiva1, Rakesh Singh2, Manish Srivastav1, Amitha Mithra S V3 and Arun Kumar M B1
1ICAR-Indian Agricultural Research Institute, India 2ICAR-National Bureau of Plant Genetic Resources, India 3ICAR-National Research Centre for Plant Biotechnology, India
ScientificTracks Abstracts: J Biotechnol Biomater
Abstract
Genetic diversity among 24 genotypes (22 varieties/collection belonging to Psidium guajava and 2 other species) of guava were characterized using Random Amplified Polymorphic DNA (RAPD) markers. Out of 29 RAPD primers used, 10 were found to be monomorphic and 19 showed polymorphism among guava genotypes. Number of alleles detected using polymorphic RAPD primer ranged between 2 (OPA13A) to 11 (OPF02A) with an average of 6 amplicons/primer. High rate of polymorphism was observed reasonably for OPF02A, OPH19A, OPF13A, OPA13A and OPB13A primers. The PIC value ranged from 0.49-0.89 indicates that the markers were quite informative. Based on molecular analysis, Sasri Selection and Sasni Selection were grouped with Allahabad Safeda. Tamil Nadu Selection and Lalit genotypes formed a group at 50% similarity. Psidum freidrichsthalianum formed a group with black guava. Molecular analysis showed a high degree of variation among analyzed guava genotypes indicating an important source of genetic diversity that can be used in the guava improvement program.Biography
Arumugam Nagaraja has completed his Master’s degree from Horticultural College and Research Institute, Periyakulam and PhD from IARI. He is the Senior Scientist in Fruit Science at the Division of Fruits and Horticultural Technology, ICAR-IARI, New Delhi. His major area of research is Guava and Mango Improvement. He has published 10 research papers in reputed national and international journals. He is also a Faculty of Postgraduate School IARI, New Delhi and engaged in Postgraduate teaching.
Email: anrciah@gmail.com
