Clinical Pharmacology & Biopharmaceutics
Open Access

Abstract

One of the frequently encountered disorders of wound healing following laryngectomy is pharyngocutaneous fistula. However, although studies have been performed with the aim of prevention of pharyngocutaneous fistulae, there are very few studies with tissue adhesives and platelet-rich plasma. In this study, our aim was to investigate the histopathologic changes in wound healing caused by various tissue adhesives and platelet-rich plasma, together with their effects on prevention of pharyngocutaneous fistula. In our published research; 40 male rats were randomly divided into five groups: Control, Platelet-rich plasma, Fibrin tissue adhesive, Protein based albumin glutaraldehyde and Synthetic tissue adhesive groups. The pharyngotomy procedure was performed and was sutured. Except the control group, tissue adhesives and platelet-rich plasma were applied. Then, the skin was sutured. On the seventh day, the rats were sacrificed. The skin was opened and pharyngotomy site was assessed in terms of fistulae. The pharyngeal suture line was evaluated histopathological by using Ehrlich Hunt scale. Inflammatory infiltration was found to be higher in platelet-rich plasma group than fibrin tissue adhesive and synthetic tissue adhesive groups. The fibroblastic activity of platelet-rich plasma, fibrin tissue adhesive and protein-based albumin glutaraldehyde groups were higher than the control group. The positive changes created by platelet-rich plasma and fibrin tissue adhesive at the histopathologic level, were together with no detected fistula. Among the study groups, there was no statistical difference for pharyngeal fistula development. This result may be obtained by the small number of the animal experiment. These results shed light on the suggestion that platelet-rich plasma and fibrin tissue adhesive can be used in clinical studies to prevent pharyngocutaneous fistula.

Biography

Email: draylineryilmaz@gmail.com