Clinical Pharmacology & Biopharmaceutics
Open Access

Abstract

Byrsonima verbascifolia (L) DC (Malpighiacea), popularly known as â��muriciâ�� is a plant that grows in the Brazilian Cerrado. The leaves of this species are widely used in folk medicine in the form of infusion to treat inflammatory conditions. The present study aims to evaluate the in vivo anti-inflammatory activity of the butanolic fraction of B. verbascifolia leaves (BvBF) in order to contribute to validation of the traditional use. The effect of the BvBF was assessed in carrageenan-induced paw edema in mice, an acute inflammation experimental model. Their chemical composition was characterized using liquid chromatography coupled to diode array detector and mass spectrometry (LC-DADâ��MS) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). All doses of BvBF tested (12.50, 25 and 50 mg/kg, intraperitoneally-i.p.) clearly demonstrate significant antiedematogenic activity (p<0.001). The suppression of local edema formation by the minor dose was 84.21�±2.236% and 53.84�±2.582%, 2 and 4 hours after the inflammatory stimulus, respectively. Moreover, BvBF (12.50 mg/kg, i.p.) also inhibited tumor necrosis factor alpha (TNF-�±) and prostaglandin E2 (PGE2) production, as well as polymorphonuclear (PMN) leucocyte recruitment to the mice footpad. Phytochemical analysis revealed the presence of the 45 compounds, including proanthocyanidins (condensed tannins), phenolic acids, flavonoids (flavones and flavonols) and other compounds. In summary, it is possible to propose that the mechanism of the anti-inflammatory action in the BvBF is linked to the decrease of pro-inflammatory mediators levels such as TNF-�± and PGE2, which leads to the inhibition of the PMN leucocyte migration.

Biography

Email: adrianasouza@ufsj.edu.br