Journal of Biotechnology & Biomaterials
Open Access

Abstract

Bioremediation of crude oil was achieved by glycolipid biosurfactant produced by marine bacterium Staphylococcus spp isolated from the petroleum hydrocarbon contaminated site cuddalore district, Tamil Nadu, India, through the standard screening and extracting procedure. The optimized physico-chemical conditions for the maximum production of biosurfactant was 30h incubation, pH 7.0, 35 º C temperature, 20ppt salinity, 3% glucose and 1.5% yeast extract as carbon and nitrogen sources respectively and ionic supplements of 0.3% K 2 HPO 4 , 0.1% FeSO 4 , 0.3% CaCO 3 , 0.2% MgSO 4 , 0.2% ZnSO 4 . Extracted partially purified biosurfactant showed broader stability over a wide range of pH 2-10, temperature up to 70ºC, salinity up to 50ppt shows the ability to withstand almost of the environmental condition and nil antimicrobial activity against bacterial and fungal strains pre-isolated from coral reef sediment and water samples shows the indigenous microorganism may not harmed therefore the fertility of the environment. Biosurfactant mediated bioremediation was carried out in conical flask using petroleum hydrocarbon pollutant and reef sediment consortium, biosurfactants solubilize the pollutants was used as sole source of carbon and energy by consortia confirmed by turbidometric, CFU and protein content in the media and was compared with the controls. From our study, isolated biosurfactant has unique property on its emulsification activity, broader stability and must provide promising environmental friendly treatment technologies for the remediation of hydrocarbons in future.

Biography

Senthil Balan S is a Research Scholar doing PhD in Marine Microbiology, Annamalai University, Tamil Nadu, India. Also holding a category as SRF in ongoing DBT project previously worked in DRDO project for a period of 2 years and totally has a research experience of 5 years. He finished his UG, PG, M.Phil and doing PhD in Microbiology in well and reputed institutes. Published 3 paper and now 3 papers in review with good impact factors. His keen interest is on bioluminescence and biosurfactant.