Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers
Google Scholar citation report
Citations : 3330
Journal of Biotechnology & Biomaterials received 3330 citations as per Google Scholar report
Indexed In
- Index Copernicus
- Google Scholar
- Sherpa Romeo
- Open J Gate
- Genamics JournalSeek
- Academic Keys
- ResearchBible
- China National Knowledge Infrastructure (CNKI)
- Access to Global Online Research in Agriculture (AGORA)
- Electronic Journals Library
- RefSeek
- Hamdard University
- EBSCO A-Z
- OCLC- WorldCat
- SWB online catalog
- Virtual Library of Biology (vifabio)
- Publons
- Geneva Foundation for Medical Education and Research
- Euro Pub
- ICMJE
Useful Links
Recommended Journals
Related Subjects
Share This Page
In Association with
Bioprospecting and rational engineering of new L-asparaginase to present a better biopharmaceutical for blood cancer treatment
Joint Event on 22nd Global Congress on Biotechnology & 5th International Conference on Enzymology and Protein Chemistry
Tales A Costa-Silva, I M Costa, G S Agamez-Montalvo, A Pessoa and G Monteiro
University of Sao Paulo, BrazilFederal University of Ceara, Brazil
Posters & Accepted Abstracts: J Biotechnol Biomater
Abstract
L-asparaginase (E.C.3.5.1.1) produced by bacteria is used in the treatment of acute lymphocytic leukemia (ALL). However, innumerable side effects were registered by the usage of bacterial L-ASNase during ALL treatment. Other drawbacks associated with prokaryotic L-asparaginase treatment are hypersensitivity reactions, low thermal stability, human proteases degradation and rapid clearance. Some techniques have been used to overcome these downsides such as bioprospecting eukaryotic sources or modification of commercial bacterial L-asparaginases by site-directed mutagenesis. In order to find eukaryotic sources of L-ASNase, 20 filamentous fungi were used in this study, which were isolated from the microbiome of the jellyfish Olindias sambaquiensis. Six fungi samples isolated from jellyfish tentacles (brown structures in jelly fish responsible to toxin production) showed L-asparaginase production by submerged fermentation process. The highest activity was shown by Strain OS02 with 2.7 U/g. This strain was selected for optimization of L-asparaginase production by central composite design of response surface methodology. For maximum enzyme production (11.45 U/g), the best condition was modified Czapek– Dox medium supplemented with L-asparagine and adjusted to pH 7.4 at 32.5 °C and 190 rpm. Regarding protein engineering of commercial bacterial L-asparaginases we used site-directed mutagenesis to obtain L-asparaginase protease-resistance: a new Escherichia coli L-asparaginase (EcAII) variant, triple mutant. The preliminary results showed that mutant enzyme was expressed in E. coli BL21 (DE3) and preserved original L-asparaginase activity. These L-asparaginases proteoforms may be alternative biopharmaceuticals with the potential of further improving outcome in ALL treatment.Biography
Tales A Costa-Silva has completed his Graduation in Biological Sciences at Federal University of Alfenas, Brazil and is pursuing his PhD at Sao Paulo University, Brazil. He has experience in Microbiology, focusing on Industrial Microbiology, acting on the following subjects: Industrial Enzymology (Production, Purification, Immobilization, Characterization and Application) and Pharmaceutical Biotechnology.
E-mail: talesmilleto@yahoo.com.br
