Journal of Analytical & Bioanalytical Techniques
Open Access

Abstract

Biophotonics is an upcoming area of photonic based emission system from biological source and utilizing this property to develop highly sensitive system for bioanalytical purpose . Light generation by the biological elements such as luciferase enzyme systems, alkaline phosphatase, horse radish peroxidase and luminol system, have numerous applications in health, food hygiene, sanitation, toxin and pathogen diagnosis. Th ese biomolecules being specifi c and highly sensitive fi nd better prospects in future in the fi eld of bioimaging, studying host pathogen interaction and developing ultrasensitive biomarkers. Th e present talk focuses on photonic based emission system for detecting food toxins and pathogens. Th e lux cassette is an array of genes that codes for bacterial bioluminescence. Recombinant bacteriophages carrying lux cassette is selective for a bacterium. Hence based upon the light emission selective bacterial pathogens can be quantifi ed. Th e antibodies against food toxins can be generated into animal models such as poultry to get IgY antibodies. Th ese antibodies have affi nity towards its corresponding toxin. Th e bio-conjugation of luciferase enzymes with these antibodies can quantify the amount of toxin present in a sample through the intensity of light emission unlike conventional ELISA based methods. Th is method is popularly known as bioluminescent immunoassay (BLEIA). Further the lux marker gene has also been inserted into certain pathogenic microbes and its pathogenicity in animal models has been studied extensively elsewhere. Th us host pathogen interaction study may fi nd immense help in drug discovery. Concluding
, biophotonics based biosensor systems has dramatically improved the present state of available diagnostic methods and is futuristic.

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