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Biodegradation of chrysene by Ochrobactrum intermedium and Enterobacter sp. isolated from the surrounding soil of Mathura oil refinery
6th World Congress on Biotechnology
Arif Ali, Asif Anas, Tanzeel, Shazia Shaheen Mir and Nimisha Patel
Jamia Millia Islamia, India
Posters-Accepted Abstracts: J Biotechnol Biomater
Abstract
Chrysene degrading bacteria were isolated from the surrounding soil of Mathura oil refinery, Uttar Pradesh, India. The strains were identified as the Ochrobactrum intermedium and Enterobacter sp. based on morphological, physiological, biochemical tests and 16s rRNA gene sequencing. Chrysene degrading bacteria from the contaminated soil were isolated by enrichment culture technique. Isolates with high chrysene degrading ability were characterized by their growth on chrysene supplemented minimal salt media. Isolated strains were incubated in MSM medium with chrysene supplemented as main carbon and energy source for seven days at 50 ppm (50 mg/l) and 100 ppm (100 mg/l) of chrysene concentration respectively. There were visible changes in the color of growth medium suggesting the degradation potential of the isolated strains and increase microbial biomass was confirmed by OD (optical density) at regular intervals. Ochrobactrum intermedium exhibited best growth (0.99 OD600) when exposed to 50 ppm and (1.07 OD600) exposed to 100 ppm of chrysene for 7 days respectively. Enterobacter sp. exhibited best growth (0.68 OD600) when exposed to 50 ppm and (0.49 OD600) exposed to 100 ppm of chrysene respectively. Above findings conclude that Ochrobactrum intermedium is more efficient in chrysene degradation than Enterobacter sp. and both the isolated strains were capable of utilizing up to 100 ppm of chrysene in MSM media.Biography
Email: patelnimisha916@gmail.com
