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Airborne microorganisms cause respiratory tract infections which spread by different sources such as droplet nuclei, dust particles,
contaminated surfaces, moist conditions, sputum spitting on floors and walls of the wards. In order to check air contamination
of different wards of Taluka Hospital, Pano Akil (District Sukkur, Sindh) both passive and active air sampling were applied according
to the standard methods. According to these results, female ward exhibited highest colony count (559.33) followed by male ward
(476), and OT (353.33), respectively. In active sampling, male ward exhibited highest colony count (564) followed by OT (430), and
female ward (372), respectively. The wards of Taluka Hospital, Pano Akil showed highest colony count and are highly contaminated as
compared to acceptable levels. The most common bacteria identified in Taluka Hospital, Pano Akil were Bacillus subtilis, Pseudomonas
aeruginosa, Staphylococcus epidermidis, Serratia marcescens, Staphylococcus aureus, and Streptobacilli. Gram positive bacteria were
found high in range as compared to Gram negative bacteria. The evidence sought in this study based on both active and passive
sampling suggests that the observed air contamination in the wards was significantly higher than the acceptable levels. Hence, it
can be deduced that the public sector hospital wards under study exhibited high level of air contamination that could be linked to
unhygienic conditions and lack of commitment. It was also observed, that the air contamination in the wards under observation
was persistent as there was no significant difference in the observed air contamination on each visit; hence the contamination was
persistently higher.