Journal of Clinical & Experimental Pathology
Open Access

Abstract

The aim of this study is to introduce a sensitiveandrapidmethodforthedetectionofpathogenicbacteria(S.typhimurium)inwater samplewasdeveloped usingbioconjugated quantum dots (CdSe/ZnS QDs)asfluorescencelabel. Salmonella typhimurium is a pathogenic Gram-negative bacteria predominately found in the intestinal lumen. Its toxicity is due to an outer membrane consistingPrimary study for the therapeutic dose and time window of picroside in treating cerebral ischemic injury in rats Yunliang Guo, Haitao Pei, Menizeng Zhang, Hui Xin, Xi Su , and Li Zhao Qingdao University Medical College, China T o explore the optimal therapeutic dose and time window of picroside for treating cerebral ischemic injury in rats according to the orthogonal test. The middle cerebral artery occlusion (MCAO) models were established by inserting intraluminally a thread into middle cerebral artery (MCA) from left external carotid artery (ECA). The successful rat models were randomly divided into sixteen groups according to the orthogonal layout of [L16(45)] and treated by injecting picroside intraperitoneally with different dose at different time. The neurological behavioral function was evaluated by Bederson?s test and the cerebral infarction volume was measured by tetrazolium chloride (TTC) staining. The expressions of neuron specific enolase (NSE) and neuroglial mark-protein S-100 were determined by immunohistochemisty assay. The results indicated that the optimal compositions of the therapeutic dose and time window of picroside in treating cerebral ischemic injury were ischemia 1.5h with 20mg/kg body weight according to Bederson?s test, 1.0h with 20mg/kg body weight according to cerebral infarction volume, 1.5h with 20mg/kg body weight according to the expressions of NSE and S-100 respectively. Based on the principle of the minimization of medication dose and maximization of therapeutic time window, the optimal composition of the therapeutic dose and time window of picroside in treating cerebral ischemic injury should be injecting picroside II intraperitoneally with 20mg/kg body weight at ischemia 1.5h. largely of lipopolysaccharides (LPS) which protect the bacteria from the environment.Surface functionalizedcore-shellquantumdots weresynthesizedbysuccessiveion layer adsorptionandreaction (SILAR)techniqueand weremade hydrophilic by ligandexchange method. DevelopedhydrophobicandhydrophilicQDswerecharacterizedbytransmission electronmicroscopy(TEM),fourier transform infra-redspectroscopy(FTIR)and spectrofluorimetry. Carboxyterminated QDs were conjugated with bacteria specific antibodies(StyphimuriumspecificIgG)forthepreparationof photostableflorescentlabelandwere characterized by various techniques like spectrofluorimetry and enzyme linked immunosorbentassay(ELISA)fortheirphotoluminescenceandsuccessful bioconjugation. Antibody(Ab)conjugatedQDswereincubatedwithbacteriacontaminatedwaterfor S.typhimuriumdetection. Microscopicimagesandspectral profileofbacteria-AbconjugatedQDscomplex were recorded by confocal laser scanning microscopy(CLSM). A sensitivity of 103organisms/mloftargetedbacteria(S. typhimurium)could beattainedina period ofabout 3hrs.

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