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Application of linear voltammetry in the determination of vitamin C in peppers
7th International Conference and Exhibition on Analytical & Bioanalytical Techniques
Hugo Romero, Carlos Garcia, Kevin Noles, Lenys Fernandes and Kerly Davila
Universidad Tecnica de Machala, Ecuador Universidad Simon Bolivar, Venezuela
Posters & Accepted Abstracts: J Anal Bioanal Tech
Abstract
The main objective of this research was to compare the content of ascorbic acid peppers (Capsicum annuum), in state of ripening green and red of the same variety. Pepper extract was obtained by crushing the fruit in a porcelain mortar, the liquid obtained was filtered on filter paper. For determining the content of ascorbic acid is used linear sweep voltammetry using three electrodes; as working electrode a platinum wire of high purity, glassy carbon as working electrode and reference electrode Ag/AgCl/KCl. A pattern of ascorbic acid and a solution of HNO3 and 0.1M NaNO3 as supporting electrolyte were used for the calibration curve. The results indicate that, green pepper has an average concentration of 1680 ppm of ascorbic acid, and red pepper reached a value of 2283 ppm. These results are superior to those obtained by Chaves et al., in pepper by HPLC. In this case, values of 1000 ppm to 1500 ppm for green pepper and red pepper were found respectively.Biography
Email: hromero@utmachala.edu.ec
