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In Association with
APPC -Antibacterial Potential of Prosopis cineraria (Linn.) Druce
6th World Congress on Biotechnology
Savita Khatri and Anil Kumar Chhillar
Maharshi Dayanand University, India
Posters-Accepted Abstracts: J Biotechnol Biomater
Abstract
Antibiotic resistance in various pathogens has exhibited an imperative need for development of novel therapeutic agents from natural resources. Since antiquity, plants have been used to treat common infectious diseases. In this regard, the antibacterial activity of medicinal plant Prosopis cineraria was investigated against Salmonella enterica ser. Typhi (MTCC-733) and Escherichia coli (MTCC-433). Five different plant extracts (petroleum ether, chloroform, acetone, methanol and water) were prepared from leaves by using Soxhlet extraction method and examined using Resazurin based Microbroth Dilution Assay (RMDA) and Disk Diffusion Assay (DDA). According to results of RMDA, acetone extract showed highest activity against S. enterica and E. coli i.e., 0.195 mg/ml and 0.390 mg/ml respectively. In DDA, acetone extract exhibited significant zone of inhibition against S. enterica and E. coli at a concentration of 75.0 �¼g per disc and 125.0 �¼g per disc respectively. Whereas, petroleum ether, chloroform, methanol and water extracts showed mild activity. Phytochemical analysis of various plant extracts revealed the presence of many secondary metabolite including tannins, steroids, flavonoids, alkaloids, glycosides, terpenoids, etc. The spectrum of activity provides possible source to obtain new and effective herbal medicines to treat infections caused by S. enterica and E. coli.Biography
Savita Khatri has completed her Graduation from Government College for Women, India and Post-graduation in Biotechnology from Centre for Biotechnology, Maharshi Dayanand University, India. She is currently pursuing her PhD from Centre for Biotechnology, Maharshi Dayanand University, India.
Email: savikhatri31@gmail.com
