Journal of Biotechnology & Biomaterials
Open Access

Abstract

Probiotics are live microorganisms, which when administered in adequate amounts confer one or more health benefits on the host by maintaining their intestinal microbial balance. A vast majority of studies in literature have dealt with the anticancer effects of probiotics on various types of cancers. The aim of present study was to investigate the antiproliferative effects of viable cells and cell-free supernatant of Pediococcus acidilactici 5101 against Human epithelial colorectal adenocarcinoma (Caco-2) and Hepatocellular carcinoma (HepG2) cell line models under in vitro conditions. MTT (3-(4, 5-dimethylthiazol- 2-Yl)-2, 5-diphenyltetrazolium bromide) cytotoxicity assay was carried out for assessing metabolic activity of the cells. Morphological abnormalities in cell lines were visualized under Scanning Electron Microscope (SEM). In concordance with previous studies, present study also revealed a dose-dependent antiproliferative effect in presence of viable cells as well as culture supernatant of P. acidilactici MTCC 5101 on cancer cell lines. Supernatant (500 �¼l/ml) had strong inhibitory effects on the growth and proliferation of cancerous cells as compared to whole viable bacterial cells as detected by colorimetric MTT assay. SEM micrographs revealed abnormalities in cell morphology after co-culturing cell lines with bacterial cells. Extensive villi disruption was observed in co-cultured samples resulting in abnormal cell morphology as compared to untreated controls. The reduction in cell division of cancerous cells by probiotics may be attributed to production of antimicrobial compounds such as bacteriocin, inactivation and inhibition of carcinogenic compounds, etc. The results emphasize on the mechanism underlying the observed antiproliferative properties of probiotic strain.

Biography

Email: tejinder.kaur.6@gmail.com