Journal of Biotechnology & Biomaterials
Open Access

Abstract

The present study was conducted to determine the antibacterial and antifungal potential of actinomycetes isolated from soil samples collected from two different locations of Punjab, India. The actinomycetes isolates showed various types of color pigments like pink, yellow, orange, red and brown which may have potential use in industries. Out of the various actinomycetes isolates, three of them showed significant inhibition of the bacteria E. coli, Staphylococcus aureus, Kleibsiella pneumonia, Salmonella enterica typhimurium and the fungi Alternaria alternata and Trichoderma viride. The crude antimicrobial compound from the best isolate A5 was obtained by ethyl-acetate extraction and the MIC against two different bacteria Klebsiella pneumoniaand Salmonellaenterica typhimuriumwas 12 mg/ml. The crude antimicrobial compound from the isolate A5 was further screened to determine the nature of the antimicrobial compound by TLC analysis. Result obtained indicated that the compound may be alcohol, phenol or steroid in nature. Further the stability of the antimicrobial compound obtained from isolate A5 was checked and it was found that the compound was stable even above 60°C and in the presence of proteinase K enzyme (5 mg/ml).The results showed that isolate A5 which produced dark orange colour pigment has excellent antimicrobial characteristics with very low MIC value and stability at high temperatures and high concentration of proteinase K.

Biography

Sandeep Rana is receiving his M.Sc. degree in Microbiology from Punjabi University, Patiala, India and is presently in his final year. His project work, under the guidance of Dr. S. Menaka Devi (Assistant Professor, Dolphin Postgraduate College of Life Sciences, Chandigarh), is on the study of actinomycetes with novel antimicrobial activity. An endeavouring young person, his area of interests is Industrial Microbiology, Medical Microbiology and Pharmacology.