Journal of Biotechnology & Biomaterials
Open Access

Abstract

Kidney stone disease is a chronic disease and its incidence has been steadily increasing for the past several decades. Proteins are found as to be the major component of kidney stone organic matrix and considered to have a key role in crystalâ�� membrane interaction, crystal growth and stone formation but their exact function in the pathogenesis of the disease still remains obscure. The present study is aimed at examining the antilithiatic potential of proteins isolated from the matrix of human kidney stones. The effect of desalted human kidney stone matrix protein extract was tested on oxalate injured Madinâ�� Darby Canine Kidney (MDCK) renal epithelial cells for their activity. The potential of kidney stone extract was assessed by cell-crystal adhesion study through imaging by phase contrast microscopy. In case of only oxalate(2mM) treated MDCK cells mostly calcium oxalate monohydrate (COM) crystals with sharp edges were observed. A comparatively lesser injury to the cells was there in case of oxalate along withkidney stone protein extract (50 �¼g/ml) treated cells as mostly calcium oxalate dihydrate (COD) crystals were observed. Although studies have shown that both COM & COD can nucleate and adhere to renal tubular epithelial cells, several lines of evidence have indicated that COM has more potent adhesive capability and can induce more toxic effects to renal tubular epithelial cells. Therefore, we can conclude that the human kidney stone matrix protein extract contains active antilithiatic proteins evincing cytoprotective activity on MDCK cells.

Biography

Email: shifa.narula11@gmail.com