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Analytical possibilities of ultra-high resolution mass spectrometry
6th International Conference and Exhibition on Analytical & Bioanalytical Techniques
Evgeny (Eugene) N Nikolaev
Institute for Energy Problems of Chemical Physics of RAS, Russia Moscow Institute of Physics and Technology (Fiztech), Russia Skolkovo Institute of Science and Technology (Skoltech), Russia
ScientificTracks Abstracts-Workshop: J Anal Bioanal Tech
Abstract
Mass spectrometry is widely used in analytical and bio analytical chemistry. Mass spectrometry of ion cyclotron resonance with Fourier Transform demonstrated the highest resolving power among other types of mass spectrometers since its opening in 1974 by Marshall and Comisarow. Recently, resolving power of FT ICR MS jumped by almost order of magnitude with introduction of the new type of FT ICR ion trap called dynamically harmonized ICR cell. Implementation of this cell on FT ICR instruments with moderate magnetic fields (5-7) Tesla permits to reach about 10 million resolving power on ion masses corresponding to mass to charge ratio m/z close to 1000 Da. With such resolving power, it is possible to resolve isotopic fine structure of molecular ions and by this to determine their atomic composition. Examples of isotopic fine structures resolution and application to analyses of crude oil, humid substances and extraterrestrial organic will be given. The initial implementation of the simple, reliable and reproducible in-ESI source H/D exchange approach coupled to ultrahigh resolution FTICR MS was performed for enumeration of labile hydrogens in individual molecules on the cluster of phosphorous acids, natural organic matter, oligosaccharides, peptides and proteins of different mass from 1 kDa to 66 kDa.Biography
Evgeny (Eugene) N Nikolaev has completed his PhD from Moscow Institute of Physics and Technology and habilitation from the Institute for Energy Problems of Chemical Physics. He is the Head of three research laboratories in Russian Academy of Sciences and Professor of Fiztech and Skoltech. He has published more than 223 papers in reputed journals and has more than 50 patents. He is the Editorial Board Member of Journal of Mass Spectrometry, European Journal of Mass Spectrometry and Rapid Communications in Mass Spectrometry. He is the Organizer of the 8th European Conference on FT ICR mass spectrometry and the First International Conference on Innovations in Mass Spectrometry Instrumentation (2013).
Email: ennikolaev@rambler.ru
