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Analysis of Linamarin compound toxicity on rubber seed oil for health disease
World Congress on Public Health and Nutrition
Bashar Mudhaffar Abdullah
University Malaya Medical Centre, Malaysia
Posters & Accepted Abstracts: J Community Med Health Educ
Abstract
In this study, lipid fraction of rubber (Hevea brasiliensis) seed was extracted and analyzed for toxicological effect. The toxicological compound such as linamarin in rubber seed oil (RSO) extracted using different solvents, such as hexane (RSOh), mixture of chloroform + methanol (RSOchl+mth) and ethanol (RSOeth) were also studied. Various methods analysis such as Fourier transforms infrared spectroscopy (FTIR), shrimps, colorimetric and rats methods were carried out to determine the present of such compounds. FTIR spectrum of RSO did not show any presence of cyanide peak. Bioassay experiments using shrimps had been used as test organisms to evaluate the toxicity of linamarin extract from RSOh, RSOchl+mth and RSOeth and LC50 were found to be (211.70 %, 139.40 %, and 117.41 %, respectively). The determination of cyanide by using colorimetric method was demonstrated no response of the cyanide in RSO and didn�t show any colored comparing with commercial cyanide which observed blue color. The results showed that no functional groups such as cyanide associated with linamarin were observed. Toxicological test using rats was also conducted to further confirm the absence of such compounds. RSO did not show any toxic potential to the rats. This can be attributed no hazardous linamarin were found in RSO.Biography
Email: bashar.mudhaffar@um.edu.my
