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In Association with
Analysis of internal transcribed spacer region of Ilex khasiana Purk and Ilex venulosa Linn, two species endemic to Meghalaya
6th World Congress on Biotechnology
Amilia Nongbet and N K Chrungoo
North Eastern Hill University, India
Posters-Accepted Abstracts: J Biotechnol Biomater
Abstract
The genus Ilex is the only living genus of family Aquifoliaceae. Sequence variation in the internal transcribed spacer (ITS) region of nuclear DNA was used to assess inter and intra-specific relationship Ilex khasiana and Ilex venulosa, two endemic tree species of Meghalaya. The nucleotide sequences of whole ITS region of I. khasiana and I. venulosa ranged between 695-697 base pairs. While ITS1 revealed highest G+C content of 60.979% and 94.75% conserved sites, ITS2 showed 29.68% variable sites with a ti/tv value of 0.51 and sequence divergence value of 11.009. The 5.8S region of nrITS was found to be the most conserved region with 99.73% conserved sites. Nucleotide sequence representing whole ITS region of I. khasiana revealed higher sequence divergence value (10.009%) than I. venulosa (5.034%). A comparative study of ITS sequences was done for the individuals representing three populations of I. venulosa. While nucleotide sequence of ITS representing individuals of population Lad-Mawphlang (E 91�°44.974�´/N 25�°22.491�´) revealed higher G+C% content of 57.020 with ti/tv value of 1.00, population Mawkajem (E 91�°53�´30.95�´�´/N 25�°20�´46.41�´�´) showed highest sequence divergence with a value of 7.286%. The neighbor-joining phylogenetic tree generated from the multiple sequence alignment of nrITS region form two separate clusters. While individuals representing I. venulosa grouped as Cluster I, individuals representing I. khasiana formed cluster II. However, one individual of I. venulosa collected from Mawkajem clustered separately as a subgroup within cluster II.Biography
Email: amilianongbet@live.com
