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Healthcare workers (HCWs) hands are the most common vehicle for the transmission of healthcare-associated pathogens from
patient to patient and within the healthcare environment. Hand hygiene is the leading measure for reducing healthcare-associated
infections (HAIs) as well as preventing antimicrobial resistance spreading. Hand rubs are gels or liquids containing antimicrobial agents
that decrease the number of microorganisms present on hands. The antimicrobial agents in most hand rubs are alcohols (ethanol,
isopropanol, and n-propanol), available in varying concentrations. A Quazi experimental, pre-post one group study was conducted
at the hematology unit, Egypt. Alcohol-based hand rubs effect on HCWs concerning the rate of HAIs among hematology patients
was evaluated. HAIs samples were collected according to site of infection and subjected to bacteriological identification. According
to the latest recommendations of the WHO, the 5 moments of hand hygiene were audited and the alcohol based hand rub personal
bottles were distributed among the HCWs to ensure maximum compliance. Moreover, direct finger prints from the HCWs dominant
hands were cultured before and after using alcohol based hand rubs. It was found that the overall percentage of HAIs in patients was
29% and 21% before and after implementation of proper hand hygiene practice among HCWs respectively. Implementation of proper
hand hygiene practice using alcohol-based hand rubs led to reduction in HAIs rate in this unit. Introduction of alcohol-based hand
rubs is key factor to overcome infrastructure barriers and build solid knowledge improvement.