Journal of Biotechnology & Biomaterials
Open Access

Abstract

Many cellular processes are accomplished through the assembly of multiple proteins acting in concert to catalyze specific activities. Although technologies for production of recombinant proteins and complexes have been tremendously improved, sample preparation often remains challenging and can require considerable investments. Many of these proteins/ complexes cannot be purified from natural source and recombinant expression from eukaryotic host is often required. Current strategies for the production of protein/complexes in insect cells using the Baculovirus expression system will be discussed. Selected examples will include vector development for parallel expression/co-expression screening, use of Lambda red recombination in E. coli for manipulation and improvement of the Baculoviral genome and assembly of multi-gene constructs from synthetic biology approaches.

Biography

Email: Arnaud.POTERSZMAN@igbmc.fr