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Cereal grains are important and rich sources of phytochemicals that have significant effects on human health. With the
growing interest in the use of valuable dietary phytochemicals, rice bran has been intensively studied for its biologically
active components. Our study investigated the relationship between carotenoid accumulation and expression of carotenoid
biosynthetic gene during the developmental stages of seed maturation in rice cultivars varying in their bran color. Transcription
levels of the key regulatory genes viz., psy pds lcy and Ã?Â? ch were higher in rice grain containing colored bran indicating that
carotogenesis is an ongoing process in mature bran. The highest level of expression and subsequent carotenoid accumulation
was noted in the purple cultivar having 44.30 �¼g/gm of beta carotene, 25.07 �¼g/gm of zeaxanthine and 2.304 �¼g/gm of lutein.
With the transition from the milking to the mature stage of grain filling in the purple cultivar, the change from ��, � carotenoid
(�±-car and lutein) accumulation to ��, �� carotenoid (��-car, zea, etc) accumulation was observed. On the other hand the brown
cultivars showed no detectable level of beta carotene, although 3.14 �¼g/gm of zeaxanthine and 6.72 �¼g/gm of lutein were noted.
To further analyze the transcription patterns of carotenoid synthesis and metabolism, the bran layer from the purple and brown
cultivars were removed. Noticeably, the purple variety showed reduced gene expression with the accumulation 0.1 �¼g/gm of
Ã?Â? carotene on the other hand, the brown cultivars showed non-detectable level of expression and carotenoid accumulation.
Transcriptome profile generated by Ion torrent further gave a better insight into the underlying mechanism that affects enzyme
synthesis/activity causing variation in the expression pattern and carotenoid content in the different developmental across
the varieties. Thus, our study indicates the potential use of purple rice as a genetic source in rice breeding programs aimed to
develop new varieties of rice that have a high content of provitamin A.
Biography
Upasna Chettry is a Junior Research Fellow availing INSPIRE Fellowship in the Department of Botany, North Eastern Hill University, Shillong. She has participated in many national conferences and symposium. She has been awarded Best presentation by PLOS at NGBT Conferences, 2014.