Journal of Fisheries & Livestock Production
Open Access

Abstract

Newcastle disease (ND) is one of the most important poultry diseases because of its widespread distribution and economic impact on poultry. The present study was conducted to estimate the longevity of thermostable ND vaccine (NDV strain I-2) in village chickens of Nepal. A total of 56 (27 days old chicks) were allocated randomly into 2 groups (treatment and control) with 28 birds in each group. On day 28, ND vaccine was administered to the treatment group only. Blood sample was collected from experimental birds at 1 day prior to vaccination and 14, 21, 30, 60, 90 and 105 days post vaccination. The serum obtained was titrated for NDV antibody using hemagglutination inhibition test. The data obtained were log transformed and subjected to the SPSS 16.0. The antibody response of treatment group revealed that the log2HI titers were 5.7, 7.0, 7.2, 6.0, 3.7 and 3.1 for 14, 21, 30, 60, 90 and 105 days after vaccination. The treatment group had higher (P<0.05) antibody titer level at 14, 21, 30, 60 and 90 days after vaccination compared to control group however, there was no significant difference in the mean titer level between treatment and control group on 105 days after vaccination suggesting that booster dose is required after 90 days of primary vaccination. Thus, thermostable ND vaccine (strain I-2) produced specific immunity against ND for at least 90 days after vaccination and may be considered suitable in Nepalese condition where cold chain maintenance is a huge challenge especially in rural area.

Biography

Email: nyaupaneshankarssp@gmail.com