Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Alcohol dependence (AD) a neuropsychiatric disorder involves complex interaction of genes and the environment leading
to a negative impact on personal, social and economic status of life. Genes of the monoamine (dopamine and serotonin)
pathways regulate the release of various neurotransmitters in the brain and studies report on an association of single nucleotide
polymorphisms (SNPs) in these genes with AD. The purpose of the study was to identify presence of SNPs in the monoamine
pathway genes in AD subjects.
Methodology: A total of 110 alcohol dependent males from the Department of Psychiatry (based on DSM-IVR criteria) and
100 healthy males from the general population (controls) formed the study group. Subjects were interviewed using the semistructured
questionnaire and WHO ASSIST and 5 ml peripheral blood was drawn after taking informed consent. Six SNPs of
dopamine pathway DRD4 120bp duplication, DRD3 Ser9Gly, DRD2 Taq1�±, COMT V158M, COMT-287A>G, DRD4 -521C/T
and two of pathway HT1B G861C and HTR3B Tyr129Ser were analyzed by PCR/RFLP. Genotype frequencies were assessed
using chi-square test and association with clinical parameters by SPSS v20.0.
Findings: Of all the studied SNPs � COMT Val158Met and HTR3B Tyr129Ser showed significant association with AD
(p=0.03). Other SNPs studied DRD4 120bp duplication (p=0.17), DRD3 Ser9gly (p=0.185), DRD2 Taq1a (p=0.27), COMT-
287 A>G (p=0.12), DRD4 -521C/T (p=0.48) did not show any association. Correlation of clinical features of the AD subjects
showed an association of HTR1B G861C with quantity of alcohol consumed per day (p=0.05) and COMT -287A/G with liver
function (SGPT p=0.04).
Conclusion and Significance: The present study is indicative of the role of COMT Val158Met and HTR3B Tyr129Ser with AD
and suggests that SNPs of the monoamine pathway genes may influence alcohol related behavior in dependent individuals.
Biography
I am Renu Singh PhD student in Department Of Anatomy, All India Institute of Medical Sciences having knowledge in molecular biology techniques such as DNA isolation, polymerase chain reaction, gel electrophoresis, restriction digestion and various other techniques. Besides that I have experience of microbiology and culture techniques.