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A Carboxylated Zn-phthalocyanine Inhibits The Fibril Formation Of Alzheimer�s Amyloid β Peptide | 21899
ISSN: 2161-0460
Journal of Alzheimers Disease & Parkinsonism
Open Access
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Amyloid β (Aβ), a 39 to 42 amino acid long peptide derives from amyloid precursor protein, is deposited as fibrils in
Alzheimer�s disease (AD) brains and considered as main cause of the disease. We have investigated the effects of a watersoluble
Zn-phthalocyanine [ZnPc(COONa)8], which have near infrared optical property, on in vitro Aβ fibril formation
process. The ThT fluorescence assay demonstrated that ZnPc(COONa)8 significantly inhibited Aβ fibril formation, as evident by
increasing the lag time and dose-dependently decreasing the fibril levels at the plateau. Moreover, it increased the destabilization
of the preformed Aβ fibrils and consequently increased monomer, dimer and trimer species of Aβ1-40 in a dose-dependent
manner. Immunoprecipitation using Aβ-specific antibody followed by near infrared scanning demonstrated the binding of
ZnPc(COONa)8 to Aβ1-42. As a result, the hydrophobicity of the Aβ1-42 fibril formation microenvironment was decreased.
Further, SDS-PAGE and dot blot immunoassay showed that ZnPc(COONa)8 delayed the reduction of low molecular weight
and appearance of higher molecular weight oligomer species of Aβ1-42. The toxicity of ZnPc(COONa)8 on the culture of a
neuronal cell line (A1) was evaluated by MTT cell viability assay. The result showed, that ZnPc(COONa)8 did not decrease
the viability, rather protected A1 cells from Aβ1-42-induced toxicity. Thus our results demonstrated that ZnPc(COONa)8
bound to Aβ and decreased the hydrophobicity of fibril formation microenvironment. This change of microenvironment
consequently inhibited oligomer and fibril formation process.
Biography
Atsushi Nagai has acquired medical doctor�s license in 1988 and completed his PhD at 1997 from Shimane Medical University and postdoctoral studies at
University of British Columbia, Canada. He is currently the Professor of Department of Laboratory Medicine at Shimane University Faculty of Medicine. He has
published more than 50 papers in reputed journals.
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