Journal of Biotechnology & Biomaterials
Open Access

Abstract

Hemorrhagic Septicemia (HS), caused by Pasteurella multocida, accounts for heavy mortality in cattle and buffalo resulting in huge economic losses to the livestock industry. The traditional method for typing Pasteurella multocida isolates by capsular and somatic typing has a low discrimination power. Hence the present investigation was conducted to characterize P. multocida isolates recovered from different species and different regions of the country over a long period of time by employing 16s rRNA gene sequencing. Twenty two isolates of P. multocida were subjected to genomic DNA isolation followed by PCR amplification of 16s rRNA gene using prokaryotic universal primers (530F and 1492R). Amplicons were then sequenced and the sequences were assembled, analyzed and compared with sequences from other reported strains. Among isolates analyzed, the similarity was varied between 91.9-100%. Sequence divergence was varied between 0-3.3% with highest divergence found between two sets of isolates viz., serotype A isolate from duck and serotype A from Goat and serotype A isolate from duck and serotype D isolate from pig. Phylogenetically, serotype A isolate from duck was highly divergent from rest of the isolates taken in the study. A total of three lineages were found among which lineage-1 comprised of serotype A isolates and lineage-2 comprised of serotype B isolates, whereas, lineage-3 comprised of A, D and F serotype isolates. These results indicated that 16s rRNA gene sequence analysis could be used for molecular characterization of isolates as well as a molecular epidemiological tool.

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