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Volume 8
Oct 25-26, 2018 Budapest, Hungary
21 st World Obesity Conference
World Obesity-2018 Journal of Obesity & Weight Loss Therapy | ISSN : 2165-7904
Beneficial effects of Ginkgo biloba extract on hippocampal oxidative
stress and serotonergic system of ovariectomized rats
Menopause is associated to the development of physical and psychological alterations
which arise in consequence to reduction of circulating estrogen levels. In cerebral
structures involved in cognition and memory as hippocampus, hypoestrogenism
may impair synaptic signaling, contributing to emergence of cognitive disturbances.
Furthermore, cognitive impairment is also associated to accumulation of oxidative
damages to different cell compounds. Ginkgo biloba extract (GbE), a widely used herbal
supplement, present many benefits associated with its antioxidant and anti-inflammatory
effects. Furthermore, we have previously demonstrated a stimulatory effect of GbE on
the hypothalamic serotonergic system of ovariectomized rats. Therefore, the aim of this
study was to evaluate GbE action on oxidative stress and 5-HT receptors expression in
hippocampus of ovariectomized female rats. 2-month-old female Wistar rats had their
ovaries surgically removed (OVX) or not (SHAM) and, sixty days after, was started the
treatment with 500 mg/Kg of GbE for 14 days. Rats were then euthanized, and hippocampi
were removed. Thereby, protein expression of 5-HT1A and 5-HT1B receptor and 5-HTT
transporter quantification were performed, as well as glutathione (GSH), superoxide
dismutase (SOD) and catalase (CAT) activities. Although no significant differences
5-HTT levels were observed, both 5-HT1A and 5-HT1B expression were diminished
in OVX rats (P=0.0471 an P=0.004, respectively) in comparison to SHAM rats, while
no differences were observed among OVX+GbE and SHAM groups. In addition, OVX
rats also presented higher SOD activity (P=0.017) in comparison to SHAM group, while
no differences were observed in the OVX+GbE group. However, no differences were
observed in GSH and CAT activities. In summary, GbE extract played an antioxidant
effect as it reduced SOD activity and also was able to protect against 5-HT1A and 5-HT1B
reduction generated in hippocampus of ovariectomized rats. These findings point to a
promising therapeutic use of GbE to improve life quality of post-menopausal women,
especially in relation to cognition.
Biography
Monica Marques Telles graduated from Mackenzie University in Sao Paulo, Brazil, with a BSc in Biological
Sciences, followed with a Master’s degree and a PhD in Nutrition from Sao Paulo Federal University in
Brazil. She currently works at Sao Paulo Federal University, Diadema Campus, being a Senior Lecturer
in General Pathology and Pathophysiology for the Undergraduate Program, and also supervises research
students in the Chemical Biology Post-graduate Program. Her area of expertise is around Human Physiology,
Pathophysiology and Pharmacology, and her research focuses on central control of food intake, hypothalamic
activity, serotonergic system, leptin secretion and activity, obesity, insulin resistance, cerebral microdialysis,
proteomic analysis, high fat diets and phytotherapic diets.
Monica Marques Telles
Federal University of São Paulo, Brazil
Monica Marques Telles et al., J Obes Weight Loss Ther 2018, Volume:8
DOI: 10.4172/2165-7904-C10-084
Co-Authors
Meira Maria Forcelini Machado,
Iracema Senna de Andrade, Valter Tadeu
Boldarine, Lila Missae Oyama,
Bruna Kelly Sousa Hirata and
Renata Mancini Banin
Federal University of São Paulo, Brazil