traditional-medicine-auckland-2018-scientifictracks

8 / 11

Page 19
Notes:
conferenceseries.com
Volume 7 Journal of Traditional Medicine & Clinical Naturopathy Traditional Medicine 2018
November 12-13, 2018
November 08-09, 2018 Auckland, New Zealand
8th International Conference and Exhibition on
Traditional & Alternative Medicine
Xiao-Lu Yin, J Tradit Med Clin Natur 2018, Volume 7
DOI: 10.4172/2573-4555-C3-008
Gynura divaricata rich in 3,5-/4,5-dicaffeoylquinic acid and chlorogenic acid restrains islet cell
apoptosis and improves pancreatic function in type-2 diabetic mice
Xiao-Lu Yin, Bing-Qing Xu and Yu-Qing Zhang
School of Biology and Basic Medical Sciences-Soochow University, China
The aim of this study was to investigate the potential protective effect of Gynura divaricate (GD) in diabetic mice. Gynura divaricata
(GD) is a kind of natural products for edible and medicine. The analytical results showed that the methanol extract of GD riches
in 10.3% 3,5- or 6.8% 4,5-dicaffeoylquinic acid and 5.6% chlorogenic acid by high-performance liquid chromatography with diode
array detection (HPLC–DAD). After STZ-induced type-2 diabetic mice (male ICR) were orally administered with 1%, 5% and 10%
GD diet for four weeks, a series of assays was performed. The hypoglycaemic rate of fasting blood glucose (FBG) reached 41.4% in
10% GD group and the fasting serum insulin in the highest dose GD group has decreased as low as normal group. GD treatment
significantly reduced the Glycosylated Serum Protein (GSP) level in diabetic mice. The activities of total Superoxide Dismutase
(SOD) and glutathione peroxidase were markedly increased; while the Malonaldehyde (MDA) level was decreased in pancreas
tissue by treatment of GD. H&E-staining showed that the pathological damage in islets with pancreatic β-cells was ameliorated
by GD treatment. An immunohistochemical assay showed that GD promoted marked β-cell regeneration. GD treatment also
caused notable increase in GLUT2, GK, MafA, PDX-1 and Bcl-2 as well as reduction in Bax and caspase-3 expression as shown
by western blot analysis. In conclusion, GD exerts the pronounced hypoglycemic effect by restraining islet cell apoptosis and
improving pancreatic function. Therefore, GD might be a promising food or medicine for the treatment of diabetes.
Biography
Xiao-Lu Yin is pursing Master’s degree in Biology from Soochow University. She has published one paper “Mulberry branch bark powder significantly improves
hyperglycemia and regulates insulin secretion in type II diabetic mice”.