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Volume 5, Issue 2 (Suppl) J Tradi Med Clin Natur
ISSN: JTMCN, an open access journal
Page 49
Notes:
Traditional Medicine 2016
September 14-16, 2016
conferenceseries.com
September 14-16, 2016 Amsterdam, Netherlands
6th International Conference and Exhibition on
Traditional & Alternative Medicine
The study of dichloromethane fraction of fruit rinds of Asam kandis (Garcinia cowa Roxb) on TNF- α
level of T47D breast cancer cell line using ELISAmethod
Fatma Sri Wahyuni, Wella Citraersya and Dessy Arisanti
Andalas University, Indonesia
Breast cancer is one of the most prevalent diseases and a major cause of death in women around the world. The rind of asam kandis
(Garcinia cowa Roxb.) contains xanthone which has potential as anticancer. In previous study, DCM fraction of the rind of asam
kandis showed cytotoxic effect on T47D breast cancer cells and also potentially inducing cell death (apoptosis) of the cancer. This
study observes how the level of TNF-α is affected by the fraction from the rind of asam kandis in T47D breast cancer cells. TNF-α is
known as an inducer of the death (apoptosis) in cells generally and as survival in tumor cell line. Levels of TNF-α are known by using
ELISA method based on the principle reaction binding between antigen and antibody. The result showed that the average levels of
TNF-α in each of control and treatment (0.1; 1.10 and 100µg/mL) were not significantly affect the levels of TNF-α with P value > 0.05.
Biography
Fatma Sri Wahyuni has completed her PhD from University of Putra Malaysia in 2010. She is the Head of Department of Pharmacy, Andalas University. She has
published more than 18 papers in reputed journals.
Fatma Sri Wahyuni et al., J Tradi Med Clin Natur 2016, 5:2 (Suppl)