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conferenceseries.com
May 01-02, 2017 Toronto, Canada
2nd International Conference on
Restorative Dentistry and Prosthodontics
Volume 5, Issue 1 (Suppl) J Oral Hyg Health
ISSN: 2332-0702 JOHH, an open access journal Restorative Dentistry & Prosthodontics 2017
May 01-02, 2017
J Oral Hyg Health 2017, 5:1 (Suppl)
Comparison of the biocompatibility of ProRoot MTA, MTAPlus and RetroMTAusing anMTT assay study
Negin Kouchak Dezfuli, Ehsan Asnaashari and Zohreh Khalilak
Tehran Medical Branch - Islamic Azad University, Iran
The purpose of this in vitro study is to evaluate and compare the cytotoxicity of three commercially available root-ending materials,
MTA Plus, Pro root MTA and Retro MTA, at different storage times after mixing on human periodontal fibroblast using a MTT
assay method. Varied concentrations (3, 6, 12, 25, 50 mg/ml) of the fresh and set root-ending materials (ProRoot MTA, MTA Plus
and Retro MTA) were placed in adjacent flasks of human periodontal fibroblast in DMEM medium within 96-well plates. Cellular
viability was evaluated using a MTT assay after 24, 48, and 72 h of initial mixing. The results were analyzed with 1-way ANOVA. The
results showed that there was no significant difference between the biocompatibility of MTA Plus, and that of Pro root MTA or Retro
MTA (P>0.05). Furthermore, no significant difference was observed between different time intervals for each group (P>0.05). The
current in vitro study showed almost similar biocompatibility for ProRoot MTA and MTA Plus and Retro MTA.