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Page 59

conferenceseries

.com

May 01-02, 2017 Toronto, Canada

2

nd

International Conference on

Restorative Dentistry and Prosthodontics

Volume 5, Issue 1 (Suppl)

J Oral Hyg Health

ISSN: 2332-0702 JOHH, an open access journal

Restorative Dentistry & Prosthodontics 2017

May 01-02, 2017

J Oral Hyg Health 2017, 5:1 (Suppl)

http://dx.doi.org/10.4172/2332-0702-C1-006

Substrate analogue targeting glutamate racemase (MurI) alters cellular morphogenesis and inhibits

biofilm formation in Streptococcus mutans

Jian-Ying Zhang

1

and

Jun-Qi Ling

2

1

Central South University, China

2

Sun Yat-sen University, China

D

-Glutamate (D-Glu) is an essential biosynthetic building block of the peptidoglycans that encapsulate the bacterial cell wall.

Glutamate racemase (MurI) catalyses the reversible formation of D-glutamate from L-glutamate and, hence, the enzyme is a

potential therapeutic target. The current study was designed to identify novel molecules that target glutamate racemase, thereby

mitigating

S. mutans

cariogenic capacities, inhibiting biofilm formation and having the potential to prevent dental caries. High

throughput screening of approximately 250 commercially available compounds against the recombinant

S. mutans

glutamate

racemase resulted in the identification of a substrate-product analogue, D-glutamine, as a modest competitive inhibitor of glutamate

racemase.

In vitro

assays, the addition of D-glutamine blocked the D-Glu metabolic way in S. mutans, leading to malformations in

bacterial cell wall, inhibition of biofilm formation, and reductions in extracellular polysaccharide (EPS) synthesis without necessarily

killing this bacterium directly. The exogenous addition of D-Glu could partially reverse the inhibitory effect of D-glutamine. In

conclusion, these findings suggest that the substrate analogue of glutamate racemase represents a promising anti-cariogenic agent in

that it suppresses virulence properties of

S. mutans

by affecting D-Glu metabolism.

zhjiangying_csu@hotmail.com