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Innovative Energy & Research | ISSN: 2576-1463 | Volume 7
Renewable Energy and Resources
Energy Materials and Fuel Cell Research
2
nd
International Conference on
&
August 27-28, 2018 | Boston, USA
Synthesis of amphiphilic fluorescent probe and follow-up imaging of Hg
2+
in living cells
Wei Gang, Gang Zhao, Shanyi Guang
and
Hongyao Xu
Donghua University, China
T
ransition metal ions have played a crucial role in the field of environment and biology, the traditional detection methods
have some limitations, low detection limit, narrow linear range and single detection. A fluorescent probe has used on
the biological and environmental analysis owning to extremely broad response range, high selectivity, real-time monitoring
capability, anti-interference and low detection limit and so on. And design and synthesis of receptor molecules with selective
recognition have attracted much attention in recent years. In this paper, rhodamine and phenyl isothiocyanate were used to
design and synthesize fluorescent probes that can efficiently detect mercury ions. It was found that the probe (ACHL) was
also able to selectively recognize Hg
2+
in DMSO/water(v/v,7:3), and detection of Hg
2+
does not disturb by the addition of
other ions and show high selectivity. The probe possesses identification stability of Hg
2+
about 3 min and displays very fast
real-time detection performance. The linear range of the probe is 1-20 uM, and the detection limit of ACHL was 0.31 uM.
Simultaneously, the probe was also applied to biological cell experiments for detection of imaging of Hg
2+
. The probe shows
good solubility in MDSO/water, lower detection limit, and well cell permeability. The cytotoxicity of the probe was measured,
found that the probes have less cytotoxic in the concentration of probe was less than 100 μM. Therefore, the probes were able
to trace intracellular Hg
2+
by fluorescence imaging in living cells.
Biography
Wei Gang is a PhD candidate in the state key laboratory for modification of chemical fibers and polymer materials, college of materials science and engineering,
Donghua University, Shanghai, China. My main research is about preparing amphipathic target fluorescent recognition molecules, and applied to specifically iden-
tify tumor cells and can track cell morphology from time to time. On the other hand, there are achievements in the field of fluorescent probes, a series of rhodamine
and fluorescein fluorescent probes were prepared and synthesized, and the cations, anions and amino acids could be specifically identified.
13171774433@163.comWei Gang et al., Innov Ener Res 2018, Volume 7
DOI: 10.4172/2576-1463-C2-006