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December 03-04, 2018 | Lisbon, Portugal
Public Health, Women's Health, Nursing and Hospital Management
Journal of Community Medicine & Health Education | ISSN : 2161-0711 | Volume 08
Joint Event
Amelia Sarmento et al., J Community Med Health Educ 2018, Volume:8
DOI: 10.4172/2161-0711-C7-049
Mycobacterium avium
paratuberculosis
persistence in drinking and
untreated water of Porto area in Portugal – A public health issue?
M
ycobacterium avium
subsp
. paratuberculosis
(MAP) has been implicated in
the development of inflammatory bowel disease (IBD) and colorectal cancer.
Portugal has a high prevalence of IBD and Porto is one of the Portuguese districts with
higher IBD prevalence.
Our goal was to assess MAP contamination in drinking and domestic untreated water
sources in Porto geographical area, since water may be an important contaminating
source of MAP for humans.
One liter of public drinking water and/or a domestic untreated water source were
collected at different locations in Porto area in early Autumn. Biofilms were also
collected by swabbing tap inner surfaces with a sterile cotton swab. A second collection
of domestic untreated water was performed after winter rains, in early February. Water
samples were filtered and DNA was extracted from both water filters and biofilm
suspensions using specific commercial kits. MAP contamination was evaluated
through a IS900-based nested PCR assay.
Drinking water samples showed higher MAP contamination than domestic water
collected in Autumn. In February, MAP detection significantly increased in domestic
water. MAP DNA was detected at a higher frequency in tap biofilms than in the
corresponding water collected.
Drinking and domestic water may be important sources of MAP contamination in
Porto area. The increased MAP detection observed after winter rains in domestic
water may reflect soil leaching. Since MAP can resist to water treatment procedures
and persist in biofilms, increased microbial surveillance and development of new
water treatment methods are most needed to avoid human exposure to this resilient
pathobiont.
Biography
Amelia Sarmento has completed her PhD at the age of 31 years from Universidade do Porto and postdoctoral
studies at Instituto de Biologia Molecular e Celular (IBMC-UP), also fromUniversidade do Porto. She is anAssociate
Professor at Faculdade de Ciências da Saúde, Universidade Fernando Pessoa at Porto and is a Researcher at
both FP-ENAS/CEBIMED (Universidade Fernando Pessoa) and at IBMC/I3S. She has published 12 papers in
reputed journals and was a GuestAssociated Editor at Frontiers Immunology/Mucosal Immunology Section, hosting
a Research Topic entitled “Understanding Crohn’s disease: immunity, genes and microbes”.
assuncao@ufp.edu.ptAmelia Sarmento
Universidade Fernando Pessoa, Portugal
Co-Authors
Telma Sousa, Jose Cabeda, Cristina Abreu
and
Conceicao Manso
University Fernando Pessoa, Portugal