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conferenceseries
.com
Volume 8
J Community Med Health Educ, an open access journal
ISSN: 2161-0711
Public Health 2018
February 26-28, 2018
PUBLIC HEALTH AND NUTRITION
3
rd
World Congress on
February 26-28, 2018 London, UK
FEASIBILITY STUDY ON MICRO-POLYETHYLENE DEGRADABILITY BY MIXED
MICROBIAL CONSORTIUM ISOLATED FROMA LANDFILL SITE
Seon-yeong Park
a
and
Chang-gyun Kim
a
a
Inha University, Korea
D
ue to increasing use and disposal of plastics or micro-bead, micro-plastics (MPs) contamination threatens the aquatic and
marine ecosystem. MPs can effect on cell viability, cell morphological change, or cause endocrine hormone disturbance
phenomena. They can also migrate long distance by local wind or ocean current, adsorbing persistent organic pollutants, heavy
metals and extraneous cell on their surface that can adverse effect on aboriginal organisms. While recent studies focused on
the distribution and ecological effect of these pollutants, there is still a comparative lack of knowledge about their biological
decomposition mechanism and biodegradability. In this study, the biodegradability of polyethylene (PE) has been investigated
by using the mesophilic mixed microbial consortium isolated from the practical landfill site in Incheon, Korea. PE is dominant
type of polymer component of MPs. PE (H(CH
2
CH
2
)nH) with a medium density of 0.94 g/mL (at 25℃) was purchased from
Sigma-Aldrich (USA). PE particles were white and amorphous granular shape sized in the range of approximately 200 to
600μm in a diameter. Mixed microbial consortia were identified as
Brevibacillus parabrevis and Paenibacillus chitinolyticus
.
For PE biodegradation test, 1 mL of mixed culture (O.D600=1.2) were inoculated in the 100 mL of freshly autoclaved Basal
medium containing 100 mg of PE as the carbon source. The cultivation was carried out at 30℃ and 150 rpm. The decomposition
was analysed through the measurement of weight loss, scanning electron microscope (FE-SEM 4300 SE, Hitachi) and fourier
transform infrared spectroscopy (FT-IR, VERTEX 80V, Bruker). After 60 days of decomposition, dry weight loss of PE was
14.7% and mean particle size decreased from 224.57μm to 175.74μm. From the SEM images, some microbes were strongly
attached on the PE surfaces verifying that they can have the potential of PE utilization.
Biography
Seon-Yeong Park has been studying soil improvement and water resource storage technologies such as evaluation of neutralization ability of acid soil and artificial
recharge technology of groundwater in the Soil and Groundwater Environment Laboratory at Inha University. She is also studying the environmental impacts and
decomposition mechanism of micro pollutants i.e., pharmaceuticals and micro plastics.
cgk@inha.ac.krSeon-yeong Park et al., J Community Med Health Educ 2018, Vol 8
DOI: 10.4172/2161-0711-C1-032