30 / 30
Page 102
conferenceseries
.com
Volume 7
Pharmaceutical Regulatory Affairs: Open Access
ISSN: 2167-7689
Pharma Europe 2018
May 07-09, 2018
May 07-09, 2018 | Frankfurt, Germany
15
th
Annual European Pharma Congress
Pharmaceut Reg Affairs 2018, Volume 7
DOI: 10.4172/2167-7689-C1-031
Electromembrane extraction combined with capillary electrophoresis for the determination of
metoclopramide and ondansetron in urine samples
Ehsan Sadeghi
and
Ali Reza Fakhari
Shahid Beheshti University, Iran
E
lectromembraneextraction(EME)isasamplepreparationtechniqueinpharmaceutical,chemical,clinicalandenvironmental
analysis. This technique uses electromigration across artificial liquid membranes for selective extraction of analytes and
sample enrichment from complex matrices. This method has many advantages such as simplicity, rapid, low-cost, low LOD,
high preconcentration factor and high recovery. In the present work, simultaneous preconcentration and determination of
two basic drugs namely metoclopramide (MCP) and ondansetron (OSN) were studied using EME as a suitable extraction
method, followed with capillary electrophoresis (CE) using ultraviolet (UV) detection as separation technique. The drugs
were extracted from 4 ml sample solutions, through a supported liquid membrane (SLM) consisting 2-nitrophenyloctylether
(NPOE) impregnated in the walls of a polypropylene hollow fiber, and into a 20μL acidic aqueous acceptor solution resent
inside the lumen of the hollow fiber with a potential difference applied over the SLM. The variables of interest, such as chemical
composition of the organic liquid membrane, stirring speed, extraction time and voltage, pH of donor and acceptor phases
and salt effect in the EME process were investigated and optimized. Under optimal conditions NPOE as SLM, stirring rate of
1000 rpm, 200V potential differences, 20min as the extraction time, acceptor phase HCl (pH 1.0) and donor phase HCl (pH
1.5). After the microextraction process, the extracts were analyzed by CE with optimum conditions phosphate running buffer
(pH 2.0), applied voltage of 20 kV and 25°C. Under the optimum conditions, limits of detection (LOD) and quantification
(LOQ) for MCP and OSNwere 2.31-2.68 and 7.72-8.91 ng mL-
1
respectively. Preconcentration factor and RSD for five replicates
of each drugs were calculated to be 200 and 4.06-3.93 respectively. Finally, the applicability of this method was studied by the
extraction and determination of these drugs in urine samples with recovery percentages of 87–92%.
sadeghiehsan88@gmail.com